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Autoantibodies can contribute to severe COVID-19 and potentially long COVID-19 symptoms

Severe coronavirus disease 2019 (COVID-19) is associated with a thrombotic inflammatory condition that results from overactivity of the immune system. New research in the United States and China.How did you investigate Autoantibodies May mediate NET during COVID-19 infection. Extensive damage to the body from post-infection neutrophil extracellular traps (NETs) can contribute to thrombotic inflammation and the symptoms experienced by COVID-19 “long-distance transporters.”

The researcher wrote:

These data reveal high levels of anti-NET antibodies in individuals admitted with COVID-19, which may impair NET clearance and thereby enhance SARS-CoV-2 mediated thrombotic inflammation. “

The study that “autoantibodies stabilize COVID-19 neutrophil extracellular traps” medRxiv* Servers and articles have been peer reviewed.

Increased anti-NET IgG and IgM antibody levels in severe COVID-19

Researchers used enzyme-linked immunosorbent assay (ELISA) to measure anti-NET IgG and IgM antibodies from sera provided by 171 patients admitted with COVID-19 and 48 healthy controls. Controls included 40 women and 8 men with an average age of 38 years. People with autoimmune diseases, active infections, or pregnancy were excluded from the study.

The team observed higher levels of anti-NET IgG and IgM in patients with COVID-19 than in controls. About 39% of patients showed high anti-NET IgG activity and 50% showed high anti-NET IgM activity. There was a strong correlation between anti-NET IgG and anti-NET IgM.

The team used an immunofluorescence microscope to evaluate anti-NET activity and found antibodies in the NET chain. There was a positive correlation between circulating markers of NET release and anti-NET IgG and IgM.

Anti-NET activity associated with serious illness

With a positive correlation between anti-NET IgG and IgM D-dimer, Absolute neutrophil count, and platelet count were observed. Similarly, there was a negative correlation between SpO2 / FiO2 ratios and anti-NET IgG and IgM.

Serum samples from patients on mechanical ventilation show higher anti-NET IgG and IgM levels, suggesting that they are associated with impaired respiratory status.

Detection of anti-NET IgG / IgM in the serum of COVID-19 patients. A, Schematic of anti-NET ELISA (created at BioRender.com). BC, anti-NET IgG and IgM were measured in sera from 171 hospitalized COVID-19 patients and 48 healthy controls. Anti-NET IgG and IgM levels at 450 nm optical density (OD) were compared by the Mann-Whitney test. **** p <0.0001. The dotted line shows the threshold set 2 standard deviations above the control mean. D, control neutrophils were stimulated with PMA to generate NET. The immobilized NET was then incubated with COVID-19 serum containing high anti-NET antibody or healthy control serum. Scale

Detection of anti-NET IgG / IgM in serum of COVID-19 patients.. A, Schematic of anti-NET ELISA (created at BioRender.com). BC, anti-NET IgG and IgM were measured in sera from 171 hospitalized COVID-19 patients and 48 healthy controls. Anti-NET IgG and IgM levels at 450 nm optical density (OD) were compared by the Mann-Whitney test. **** p <0.0001. The dotted line shows the threshold set 2 standard deviations above the control mean. D, control neutrophils were stimulated with PMA to generate NET. The immobilized NET was then incubated with COVID-19 serum containing high anti-NET antibody or healthy control serum. Scale bar = 100 microns.

Autoantibodies impair the ability of serum DNases to clear NETs

The next step was to assess the role of autoantibodies in NET degradation. They measured IgG levels in four patients with COVID-19 high in anti-NET IgG and tested with healthy control sera. The results showed that high IgG levels prevented NET degradation.

Given the reduced oxygen uptake, increased risk of serious illness, and impaired NET degradation, researchers show that autoantibodies play an important role in the long-term damage suffered by people with long-lasting COVID. It suggests that there is a possibility.

These anti-NET antibodies may be important orchestrator of the imbalance between NET formation and the clearance that persists COVID-19 thrombotic inflammation, “the research team concluded.

*Important Notices

medRxiv Publish preliminary scientific reports that should not be considered definitive as they are not peer-reviewed, guide clinical practice / health-related behaviors, and should not be treated as established information.

Autoantibodies can contribute to severe COVID-19 and potentially long COVID-19 symptoms

Source link Autoantibodies can contribute to severe COVID-19 and potentially long COVID-19 symptoms

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