Histology is the study of living tissue at the microscopic level. Histology, also known as microscopic anatomy, is widely used in the diagnosis of cancer and other diseases. For example, tissue samples obtained during surgery may help determine if further surgery is needed, and if a diagnosis can be obtained quickly during surgery, further surgery can be avoided. I can do it.
Traditional methods in histopathology are generally limited to thin specimens and require chemical treatment of the tissue to provide sufficiently high contrast for imaging, slowing down the process. Recent advances in histopathology have eliminated the need for chemical staining and enabled high-resolution imaging of thick tissue sections.As reported in Advanced photonics, An international research team has recently demonstrated a 3D label-free quantitative phase imaging technique that uses optical diffraction tomography to acquire volumetric imaging information. Automatic stitching simplifies image acquisition and analysis.
Optical diffraction tomography
Optical diffraction tomography is a microscopic technique for reconstructing the index of refraction of tissue samples from scatter field images obtained at different illumination angles. Allows label-free, high-contrast visualization of transparent samples. The complex scattering field that has passed through the sample is first obtained using off-axis holography, and then the scattering fields obtained at various illumination angles are mapped to Fourier space, allowing the index of refraction of the sample to be reconstructed. Will be.
The perceived limitation of photodiffraction tomography is due to the complex distribution of the index of refraction, which results in significant optical aberrations in the imaging of thick tissues. To overcome this limitation, the team used digital refocusing and automatic stitching to enable 100-volume imaging. m-Tissue with a lateral field of view of 2 mm thick 1.75mm while maintaining high resolution of 170nm 170nm 1400nm. They demonstrated that simultaneous visualization of intracellular and mesoscopic structures in different tissues is possible with high resolution combined with a wide field of view.
Fast and accurate histopathology
Researchers have demonstrated the potential of new methods by imaging the pathology of a variety of cancers, including pancreatic neuroendocrine tumors, intraepithelial tumors, and intraductal papillary tumors of the bile ducts. They imaged unstained 100 μm thick tissue on the millimeter scale at intracellular 3D resolution. This allows visualization of individual cell and multicellular tissue structures, comparable to images obtained with traditional chemically treated tissue.
Yong Kuen Park, a researcher at the Korea Institute of Advanced Science and Technology and the lead author of the research, said: Medical condition. “
Volumetric histopathology of an unlabeled 100 μm thick pancreatic tissue sample from a patient with intraductal papillary neoplasm of the bile duct of the liver. For comparison, adjacent tissues were prepared into thin tissue slides using conventional H & E staining. (5th line, magnification 400 times). Image credit: Hugonnet et al. , Doi 10.1117 / 1.AP.3.2.026004.
Park states that further research is needed, but the results suggest great potential for rapid and accurate histopathology during surgery. Accurate diagnosis in histopathology and intraoperative pathology consultation. “
Hugonnet, H. , et al. (2021) Multiscale label-free volume holographic histopathology of tissue slides with high intracellular resolution. Advanced photonics. doi.org/10.11 17 / 1.AP.3.2.026004..
New methods provide faster and more accurate diagnosis in histopathology
Source link New methods provide faster and more accurate diagnosis in histopathology